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Dot blot is a technique for detecting, analyzing, and identifying proteins. This technique is similar to western blot, but protein samples are not separated using electrophoresis; instead, proteins are spotted through circular templates directly onto the membrane or paper substrate.
The concentration of proteins in crude preparations (such as culture supernatant) can be estimated semi-quantitatively using the dot blot method if you have both purified protein and the specific antibody against it. In this case, compare the signal from the unknown sample to that of the standard and estimate the concentration.
